Israël Noot was the son of Philip Noot and Schoontje Leijden van Amstel. He was married to Debora Busnach. They got engaged in 1939 in Amsterdam. At that time he lived with his parents in the Spitskopstraat 5II.
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Israël Noot worked as an assistant at the Pedagogium Achisomog of the Apeldoornse Bos, where he started working on 11 February 1940.